Neurofarmacología

Laboratorio de Neurofarmacología

Laboratory of Neuropharmacology

INVESTIGADOR RESPONSABLE

Dra. Laura Ruth Guelman

Investigadora independiente CONICET

Información del Laboratorio

Línea de Investigación

Alteraciones conductuales inducidas por la exposición al ruido y la ingesta de etanol en diferentes etapas del desarrollo en un modelo murino. Mediadores bioquímicos y moleculares en el Sistema Nervioso Central y estrategias de neuroprotección.

Dada la prevalencia del consumo de alcohol con fines recreativos entre los adolescentes humanos, así como su uso en entornos de entretenimiento caracterizados por altos niveles de ruido, el uso de un modelo animal adolescente apropiado para evaluar los efectos de estos agentes ambientales sobre el Sistema Nervioso Central (SNC), resulta clínicamente relevante.

Nuestra investigación tiene como objetivo evaluar el impacto de la ingesta de etanol y la exposición al ruido, tanto individual como secuencialmente, sobre comportamientos similares a la ansiedad y varios tipos de memoria, los cuales dependen de la integridad de una estructura del SNC, el hipocampo.

Además, nos centramos en investigar los mecanismos moleculares y los mediadores bioquímicos que pueden sustentar estas posibles alteraciones, con un énfasis particular en el estrés oxidativo y en la neurotransmisión aminoacidérgica.

Finalmente, proponemos estudiar la efectividad del ambiente enriquecido como estrategia de neuroprotección no farmacológica en este modelo, lo cual podría contribuir a establecer las bases necesarias destinadas a proponer potenciales intervenciones terapéuticas en humanos, pudiendo utilizarse el ambiente enriquecido como una intervención complementaria al tratamiento farmacológico de enfermedades neuropsiquiátricas y lesiones cerebrales.

Recientemente, iniciamos una nueva línea de investigación en adolescentes y adultos emergentes humanos, con el objetivo de conocer qué factores favorecen o previenen el consumo de alcohol en la población del Área Metropolitana de Buenos Aires (AMBA). Evaluamos la cantidad y la frecuencia del consumo de alcohol en esta población y estudiamos posibles factores asociados como la presencia de estresores y un estilo de vida saludable.

Behavioral alterations induced by noise exposure and ethanol intake at different developmental stages in a rodent model. Biochemical and molecular mediators in the Central Nervous System and neuroprotection strategies.

Given the prevalence of recreational alcohol consumption among human adolescents, as well as their use in entertainment environments characterized by high noise levels, the use of an appropriate adolescent animal model to evaluate the effects of these environmental agents on the Central Nervous System (CNS) is clinically relevant.

Our research aims to evaluate the impact of ethanol intake and noise exposure, both both individually and sequentially, on anxiety-like behaviors and various types of memory, which depend on the integrity of a CNS structure, the hippocampus.

In addition, we focused on investigating the molecular mechanisms and biochemical mediators that may underlie these potential alterations, with a particular emphasis on oxidative stress and the aminoacidergic neurotransmission.

Finally, we propose to study the effectiveness of the enriched environment as a non-pharmacological neuroprotection strategy in this model, which could contribute to establish the essential basis for proposing potential therapeutic interventions in humans, whereby the enriched environment could be used as a complementary intervention to the pharmacological treatment of neuropsychiatric diseases and brain injuries.

Recently, we initiated a new line of research in human adolescents and emerging adults, with the aim of finding out what factors favor or prevent alcohol consumption in the population of the Buenos Aires Metropolitan Area (AMBA). We evaluated the amount and frequency of alcohol consumption in this population and studied possible associated factors such as the presence of stressors and a healthy lifestyle.

Convenios y Actividades

Convenios y Actividades

Tesis Doctorales (rendidas desde 2012 a la fecha):

Lucila Guadalupe Caceres: diciembre de 2013

Soledad Lucía Uran: Septiembre de 2015

Sonia Jazmín Molina: Marzo de 2019

Gustavo Ezequiel Buján: Octubre de 2023

Actividad Docente:

Laura Ruth Guelman: Jefe de Trabajos Prácticos y Docente autorizada de Farmacología, Facultad de Medicina (UBA)

Sonia Jazmín Molina: Ayudante de 1ra de Neurofisiología, Facultad de psicología (UBA)

Lara Candela Araujo Añon: Ayudante de 2da de Neurofisiología, Facultad de psicología (UBA)

Asesorías:

Invitación en carácter de Asesora Técnica a la Honorable Cámara de Diputados de la Nación Argentina (reunión de asesores de la Comisión de Recursos Naturales) para el tratamiento de la Ley de Presupuestos Mínimos para el Control de la Calidad Acústica, abril 2016 (sancionada en 2017).

Publicaciones

Buján, G.E., D´Alessio, L., Serra, H.A., Molina, S.J., Guelman, L.R. BEHAVIOURAL ALTERATIONS INDUCED BY INTERMITTENT ETHANOL INTAKE AND NOISE EXPOSURE IN ADOLESCENT RATS, Eur J Neurosci. 55(7):1756-1773, 2022. Available online, April 2022, doi: 10.1111/ejn.15657.
Molina, S.J., Guelman, L.R. NOISE-INDUCED HIPPOCAMPAL DAMAGE: POTENTIAL MECHANISMS. Neural Regen. Res, 17(3):563-564, 2022. Available online, August 2021, 10.4103/1673-5374.320982.
Molina, S.J., Lietti, A.E., Carreira Caro, C.S., Buján, G.E., Guelman, L.R. EFFECTS OF EARLY NOISE EXPOSURE ON HIPPOCAMPAL-DEPENDENT BEHAVIORS DURING ADOLESCENCE IN MALE RATS: INFLUENCE OF DIFFERENT HOUSING CONDITIONS, Animal Cognition 2022, 25(1):103-120. Available online, July 2021, 10.1007/s10071-021-01540-1.
Molina, S.J., Buján, G.E., Guelman, L.R. NOISE-INDUCED HIPPOCAMPAL OXIDATIVE IMBALANCE AND AMINOACIDERGIC NEUROTRANSMITTERS ALTERATIONS IN DEVELOPING MALE RATS: INFLUENCE OF ENRICHED ENVIRONMENT DURING ADOLESCENCE. Dev. Neurobiol. 2021, 81(2):164-188. Available online, December 2020, 0.1002/dneu.22806.
D´Alessio, L., Korman, G.P., Sarudiansky, M., Guelman1, L.R., Scévola, L. Pastore, A., Obregón, A., Roldán, E.J. REDUCING ALLOSTATIC LOAD IN DEPRESSION AND ANXIETY DISORDERS: PHYSICAL ACTIVITY AND YOGA PRACTICE AS ADD-ON THERAPIES. Front. Psychiatry 2020, 11:501. Available online, june 2020, 10.3389/fpsyt.2020.00501.
Buján, G.E., Serra, H.A., Molina, S.J., Guelman, L.R.. OXIDATIVE STRESS-INDUCED BRAIN DAMAGE TRIGGERED BY VOLUNTARY ETHANOL CONSUMPTION DURING ADOLESCENCE: A POTENTIAL TARGET FOR NEUROPROTECTION?. Curr. Pharm. Design 2019, 25 (1-7). Available online, December 2019, 10.2174/1381612825666191209121735.
Molina, S.J., Buján, G.E., Rodriguez Gonzalez, M., Capani, F., Gómez-Casati, M.E., Guelman, L.R. EXPOSURE OF DEVELOPING MALE RATS TO ONE OR MULTIPLE NOISE SESSIONS AND DIFFERENT HOUSING CONDITIONS: HIPPOCAMPAL THIOREDOXIN CHANGES AND BEHAVIORAL ALTERATIONS. Front. Behav. Neurosci. 2019, 13:182. Available online, august 2019, 10.3389/fnbeh.2019.00182.
Miceli, M., Molina, S.J., Forcada, A., Acosta, G.B., Guelman, L.R. VOLUNTARY ALCOHOL INTAKE AFTER NOISE EXPOSURE IN ADOLESCENT RATS: HIPPOCAMPAL-RELATED BEHAVIORAL ALTERATIONS. Brain Res. 2018, 1679: 10-18. Available online, November 2017, 10.1016/j.brainres.2017.11.001.
Saraceno, G.E., Caceres, L.G., Guelman, L.R., Castilla, R., Udovin, L.D., Ellisman, M.H., Brocco, M.A., Capani, F. CONSEQUENCES OF EXCESSIVE PLASTICITY IN THE HIPPOCAMPUS INDUCED BY PERINATAL ASPHYXIA. Exp. Neurol. 2016, 286:116-123. Available online, August 2016.
Molina, S.J., Capani, F, Guelman, L.R. NOISE EXPOSURE OF IMMATURE RATS CAN INDUCE DIFFERENT AGEDEPENDENT EXTRA-AUDITORY ALTERATIONS THAT CAN BE PARTIALLY RESTORED BY REARING ANIMALS IN AN ENRICHED ENVIRONMENT. Brain Res. 2016, 1636:52-61. Available online, February 2016.
Molina, S.J., Miceli, M., Guelman, L.R. NOISE EXPOSURE AND OXIDATIVE BALANCE IN AUDITORY AND EXTRA-AUDITORY STRUCTURES IN ADULT AND DEVELOPING ANIMALS. PHARMACOLOGICAL APPROACHES AIMED TO MINIMIZE ITS EFFECTS. Pharmacological Res., 2016, 109:86-91. Available online, December 2015.
Romero, J, Muñiz, J, Tornatore, TL, Holubiec M, González J, Barreto GE, Guelman L, Lillig CH, Blanco E, Capani F. DUAL ROLE OF ASTROCYTES IN PERINATAL ASPHYXIA AND NEUROPROTECTION. Neurosci Lett. 2014, 565:42-6
Long-term recovery from hippocampal-related behavioral and biochemical abnormalities induced by noise exposure during brain development. Evaluation of auditory pathway integrity.
Uran SL, Gómez-Casati ME, Guelman LR.
Int J Dev Neurosci. 2014 Oct;37:41-51.
Pharmacological alterations that could underlie radiation-induced changes in associative memory and anxiety.
Caceres LG, Cid MP, Uran SL, Zorrilla Zubilete MA, Salvatierra NA, Guelman LR.
Pharmacol Biochem Behav. 2013 Oct;111:37-43.
Immune alterations induced by chronic noise exposure: comparison with restraint stress in BALB/c and C57Bl/6 mice.
Pascuan CG, Uran SL, Gonzalez-Murano MR, Wald MR, Guelman LR, Genaro AM.J Immunotoxicol. 2014 Jan-Mar;11(1):78-83.
Rat hippocampal alterations could underlie behavioral abnormalities induced by exposure to moderate noise levels.
Uran SL, Aon-Bertolino ML, Caceres LG, Capani F, Guelman LR.
Brain Res. 2012 Aug 30;1471:1-12.
Carreira Caro, C.S., Lietti, A.E., Buján, G.E., Formoso, J., Pilatti, A., Guelman, L.R., Molina, S.J. PHYSICAL ANDSOCIAL DRINKING CONTEXTS CHANGES DUE TO THE LOCKDOWN DURING THE COVID-19 PANDEMICMODIFIED ALCOHOL CONSUMPTION IN A SAMPLE OF ARGENTINE COLLEGE STUDENTS, J Drug Issues, InPress. Available online, January 2024, doi: 10.1177/00220426241235159.